rabbit anti sheep ab conjugated to fitc  (Vector Laboratories)

Journal:

Article Title: BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma

doi: 10.1073/pnas.092123699

Figure Lengend Snippet: Mutually exclusive expression patterns of BORIS and CTCF correlate with epigenetic reprogramming in testis. (A and E) Immunostaining of thin (5-mm) sections of adult mouse testis with the affinity-purified anti-BORIS antibodies visualized by secondary alkaline phosphatase-conjugated rabbit anti-IgY antibodies. (B and F) Expression in adult mouse testis sections visualized using affinity-purified anti-N-CTCF antibodies and secondary alkaline phosphatase-conjugated antibodies. (C and D) Control staining obtained with normal chicken and rabbit serum, respectively. (G) BORIS-expressing cells in mouse tubule visualized by secondary FITC-labeled antibodies. (H) 4′,6-diamidino-2-phenylindole (DAPI)-inverted image of G. (I) Dual immunohistochemical staining by chicken ap-2-Ab anti-BORIS antibodies (Rhodamine fluorescence, red) and anti-5mC antibodies (FITC fluorescence, green). (J) Anti-5mC staining (FITC with DAPI counterstaining) at lower magnification. (K) Identification of the cell types with methylated DNA within the tubule, SG, Sertoli cells, and St. Note methylation free Sc. (L) An inverted DAPI image of K. (Magnification: A–D, 12-fold; E and F, 50-fold; G, H, K, and L, 106-fold; I and J, 44-fold.)

Article Snippet: For double-staining of chicken anti-BORIS and sheep anti-5mC Ab, a goat anti-chicken secondary Ab conjugated to biotin (Vector), with subsequent detection by avidin-Rhodamine (Vector), was used for BORIS detection, and rabbit anti-sheep Ab conjugated to FITC (Vector) for detection of 5mC.

Techniques: Expressing, Immunostaining, Affinity Purification, Staining, Labeling, Immunohistochemical staining, Fluorescence, Methylation